Table 2. FOXO3a-based characterization of parental cell lines and their proliferative response to glucose-modified PacR cancer cell secretome.
|
+Glucose (+25 mM glucose) |
−Glucose (glucose-deprived) |
|||||||
|---|---|---|---|---|---|---|---|---|
|
Cell proliferation (%±s.d., 48 h rel. 24 h) |
Cell proliferation (%±s.d., 48 h rel. 24 h) |
|||||||
| Fresh medium |
PacR secretome |
PacR secretome |
||||||
| Phenotype description | Cell line ID | FOXO3a WB grade | siControl | siFOXO3a | Fresh medium | siControl | siFOXO3a | |
| Parent | A549 | a | 60.1±0.6 | 58.5±2.1 | 42.1±1.4 | 28.1±4.2 | 29.4±4.2 | 9.3±6.8 |
| Paclitaxel resistance (PacR) | A549-PacR | b | 83±1.67 | 79.3±3.9 | 58.1±2.2 | 33.0±3.3 | 30.8±2.1 | 12.1±2.1 |
| Transient cross-resistant PacR | A549-PacR/DCT | b | 112.3±0.5 | 110.2±0.3 | 85.8±7.1 | 41.0±0.9 | 37.7±1.6 | 18.6±3.2 |
| Parent | PC-3 | a | 54.2±1.2 | 51.3±1.3 | 31.3±8.2 | 22.1±1.8 | 20.1±2.9 | 10.2±1.1 |
| Paclitaxel resistance (PacR) | PC-3-PacR | b | 69.1±0.4 | 66.5±1.5 | 29.6±3.3 | 31.2±1.7 | 29.1±2.2 | 15.9±0.8 |
| Transient cross-resistant PacR | PC-3-PacR/DCT | c | 89.7±2.2 | 82.1±2.1 | 54.4±5.2 | 29.2±3.2 | 31.3±0.5 | 15.2±2.1 |
Abbreviation: WB, western blot.
a
Low level expression.
b
Medium level expression.
c
rel., relative to.