FIG. 2.
Effect of different concentrations of vancomycin on the proportion of the UDP-MurNAc-pentapeptide and UDP-MurNAc-depsipeptide in the cell wall precursor pool. Strain COLVA200 was grown in antibiotic-free medium and with medium supplemented with different concentrations of vancomycin. The analysis of the cell wall precursor pool was performed as described in Materials and Methods. For comparison, the composition of the cell wall precursor pool was also determined in COLVAORG. UDP-MurNAc-pentapeptide, empty bars; UDP-MurNAc-depsipeptide, solid bars.