FIG. 5.

Effect of the transcription of pbp2 on the composition of cell wall peptidoglycan produced in S. aureus strain COLVA₂₀₀. COLVA₂₀₀pPBP2iC, in which pbp2 was put under the control of the inducible promoter spac, was grown without and with an optimal concentration of IPTG inducer (500 μM). Additional variants included the presence of vancomycin in the growth medium. The top two panels show the HPLC profile of muropeptides in the peptidoglycan of COLVA₂₀₀ and in the peptidoglycan of the same strain grown in the presence of 10 μg of vancomycin/ml. Panels 3, 4, and 5 show the effect of IPTG and vancomycin on the structure of the cell wall peptidoglycan.