TABLE 2.
Muropeptide composition of major peaks of vacomycin-resistant S. aureus strain COLVA
Major peaka | % of compositionb
|
|||||
---|---|---|---|---|---|---|
COLVAORG | COLVAORG + (10 μg/ml) vancomycin | COLVA200 | COLVA200 + (10 μg/ml) vancomycin | COLVA200Tn551::mecA | COLVA200Tn551::mecA + (10 μg/ml) vancomycin | |
1 | 1.7 | 0.0 | 0.4 | 0.0 | 2.1 | 0.0 |
5 | 2.9 | 0.0 | 1.3 | 0.0 | 0.5 | 0.0 |
11 | 4.3 | 0.0 | 1.9 | 0.0 | 1.6 | 0.0 |
15 | 4.2 | 0.0 | 2.3 | 0.0 | 1.8 | 0.0 |
16 | 3.2 | 0.0 | 2.1 | 0.0 | 1.9 | 0.0 |
17 | 3.0 | 0.0 | 3.5 | 0.0 | 2.9 | 0.0 |
3v | 0.5 | 10.0 | 1.7 | 10.0 | 0.4 | 12.4 |
8v | 1.7 | 2.3 | 3.1 | 2.3 | 3.3 | 8.9 |
11v | 0.5 | 1.9 | 1.2 | 1.9 | 1.2 | 3.1 |
13v | 1.7 | 3.4 | 4.9 | 3.4 | 5.2 | 7.7 |
14v | 0.6 | 1.9 | 1.3 | 1.9 | 1.2 | 2.7 |
15v | 1.2 | 3.4 | 4.4 | 3.4 | 4.4 | 5.7 |
16v | 0.0 | 1.4 | 1.2 | 1.4 | 0.9 | 1.3 |
17v | 0.8 | 2.4 | 2.6 | 2.4 | 2.6 | 2.8 |
19v | 0.0 | 1.4 | 1.2 | 1.4 | 1.2 | 1.8 |
Monomers | 13.9 | 21.9 | 14.2 | 21.9 | 14.0 | 35.3 |
Oligomers | 47.6 | 40.4 | 51.6 | 40.4 | 46.4 | 48.8 |
Hump | 37.6 | 38.3 | 32.8 | 38.3 | 39.0 | 15.8 |
Muropeptide peak numbers as in Fig. 2.
Relative amounts of muropeptide species are expressed as the percent of total UV-absorbing material recovered from the HPLC column.