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. 2004 Dec;48(12):4654–4661. doi: 10.1128/AAC.48.12.4654-4661.2004

TABLE 3.

Steady-state kinetic parameters of purified GIM-1 compared to those of IMP-1, VIM-1, VIM-2, and SPM-1

Antibiotic	Kinetic parameters^a of:
	GIM-1^b			IMP-1^c			VIM-1^d			VIM-2^e			SPM-1^f
	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ s⁻¹)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ s⁻¹)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ s⁻¹)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ s⁻¹)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ s⁻¹)
Penicillin	6.6	46	0.14	320	520	0.62	29	841	0.034	55.8	49	1.14	108	38	2.8
Ampicillin	3.3	20	0.16	950	200	4.8	37	917	0.04	-	-	-	117	72	1.6
Carbenicillin	4.1	170	0.02	ND	ND	0.02	167	75	2.2	-	-	-	74	814	0.09
Azlocillin	ND	ND	ND	-	-	-	1,525	123	12	-	-	-	53	147	0.35
Piperacillin	6.9	69	0.10	ND	ND	0.72	1,860	3,500	0.53	32.7	72	0.45	117	59	2
Ticarcillin	2.3	57	0.04	1.1	740	0.0015	452	1,117	0.41	31.7	46	0.69	ND	<0.35	ND
Nitrocefin	5.8	12	0.47	63	27	2.3	95	17	5.6	-	-	-	0.53	4	0.12
Cephalothin	16	22	0.72	48	21	2.4	281	53	5.3	56.2	44	1.28	43	4	11.7
Cefuroxime	5.9	7	0.80	8	37	0.22	324	42	7.7	12.1	22	0.55	37	4	8.8
Cefoxitin	8.3	206	0.04	16	8*	2	26	131	0.2	3	24	0.12	8	2	4
Ceftazidime	18	31	0.58	8	44	0.18	60	794	0.076	89	98	0.90	28	46	0.6
Cefotaxime	1.1	4	0.24	1.3	4*	0.35	169	247	0.68	27.5	32	0.86	16	9	1.9
Cefepime	17	431	0.04	7	11*	0.66	549	145	3.8	4.7	184	0.03	18	18	1
Imipenem	27	287	0.09	46	39	1.2	2.0	1.5	1.3	9.9	10	0.99	33	37	1
Meropenem	2.7	25	0.11	50	10	0.12	13	48	0.27	1.4	5	0.28	63	281	0.22
Moxalactam	14	1,035	0.01	88	10*	8.8	-	-	-	14.8	80	0.18	13	97	0.13
Aztreonam	ND	ND	ND	>0.01	>1,000	<10⁻⁵	<0.01	>1,000	<10⁻⁵	<0.5	ND	ND	ND	<0.3	ND
Clavulanic acid	ND	ND	ND	-	-	-	-	-	-	-	-	-	ND	>0.1	ND
Tazobactam	ND	ND	ND	>1,000	>3.98	0.0039	5.3	337	0.016	-	-	-	0.6	3	0.2

^a

ND, data could not be determined; -, data not available; *, K_m was obtained as the K_i value.

^b

As found in the present study.

^c

Laraki et al. (14).

^d

Franceschini et al. (7).

^e

Poirel et al (21).

^f

Murphy et al. (18).