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. 2004 Dec;48(12):4643–4649. doi: 10.1128/AAC.48.12.4643-4649.2004

FIG. 3.

FIG. 3.

SDS-12.5% polyacrylamide gel showing steps involved in purification of B. anthracis Sterne rDHFR. Lanes 1 and 6 contain Novagen Perfect Protein markers (15 to 150 kDa ladder). Lane 2 contains cell lysate from E. coli BL21 Star (DE3) carrying plasmids pLysS and pCRT7/CT-TOPO-Sterne rDHFR with the six-histidine tag (precolumn) (lysate filtered through a 0.22-μm-pore-size filter). Lane 3 contains pooled Sterne rDHFR with the six-His tag (enzyme purified by passage through a nickel column). Lane 4 contains FPLC-purified Sterne rDHFR with the six-His tag, and lane 5 contains FPLC-purified Sterne rDHFR with the six-His tag removed.