Fig. 3.

Effect of blocking α2AP on the systemic sclerosis (SSc) dermal fibroblast-induced vascular dysfunction in endothelial cells (ECs). a ECs were seeded on 96-well Matrigel coated plates. ECs were cultured with the condition media (CM) of human normal dermal fibroblasts or human SSc dermal fibroblasts for 24 hours. b Tube formation in ECs was measured as described in Materials and Methods (n = 6). c ECs were cultured with the CM of the human normal dermal fibroblasts or the human SSc dermal fibroblasts for 24 hours. The cell proliferation was assessed as described in Materials and Methods (n = 3). d ECs were cultured with the CM of human normal dermal fibroblasts or human SSc dermal fibroblasts for 24 hours. The expression of each protein was examined by western blot analysis. e ECs were seeded on 96-well Matrigel-coated plates. ECs were cultured with the CM of human SSc dermal fibroblasts, and were stimulated by control IgG (1 μg/mL) or α2AP-neutralizing antibodies (1 μg/mL) for 24 hours. f Tube formation in ECs was measured as described in Materials and Methods (n = 6). g ECs were cultured with the CM of the human SSc dermal fibroblasts, and were stimulated by control IgG (1 μg/mL) or α2AP-neutralizing antibodies (1 μg/mL) for 24 hours. Cell proliferation was assessed as described in Materials and Methods (n = 8). h ECs were cultured with the CM of human SSc dermal fibroblasts and were stimulated by control IgG (1 μg/mL) or α2AP-neutralizing antibodies (1 μg/mL) for 24 hours. The expression of each protein was examined by western blot analysis. Data represent the mean ± SEM. * P < 0.01, ** P < 0.05. Scale bar, 50 μm