TABLE 2.
Bactericidal activity of hLF(1-11) peptide against various A. baumannii strainsa
| Peptide concn (μM) | No. of surviving organisms (CFU/ml)
|
||||
|---|---|---|---|---|---|
| RUH 875 | LUH 7858 | RUH 134 | LUH 7213 | LUH 6034 | |
| hLF(1-11) | |||||
| 0 | 12 × 105 ± 4 × 105 | 9 × 105 ± 2.1 × 105 | 1.2 × 106 ± 0.1 × 106 | 6 × 105 ± 4.4 × 105 | 2 × 106 ± 0.4 × 106 |
| 9 | 7 × 104 ± 8.9 × 104b | 9 × 104 ± 8.8 × 104b | 3 × 104 ± 1 × 104b | 3 × 104 ± 3 × 104b | 8 × 104 ± 4.8 × 104b |
| 18 | 4 × 103 ± 3.2 × 103b | 3 × 104 ± 3.2 × 104b | 5 × 103 ± 2.9 × 103b | 2 × 103 ± 2 × 103b | 15 × 103 ± 3.8 × 103b |
| 36 | 2 × 103 ± 1.4 × 103b | 4 × 103 ± 2.9 × 103b | 3 × 103 ± 1.4 × 103b | 12 × 102 ± 5.7 × 102b | 4 × 103 ± 2.7 × 103b |
| 72 | 12 × 102 ± 7.5 × 102b | 63 × 102 ± 3.8 × 102b | 10 × 102 ± 6.2 × 102b | 670b | 670b |
| Control peptide | |||||
| 72 | 8 × 105 ± 1.4 × 105 | 6 × 105 ± 2.1 × 105 | 2 × 106 ± 1.2 × 106 | 8 × 105 ± 4.8 × 105 | 1 × 106 ± 1.2 × 106 |
a
Approximately 1 × 106 CFU of A. baumannii was incubated for 1 h at 37°C with a range of concentrations of hLF(1-11) (0 to 72 μM) or with 72 μM control peptide, and then the number of surviving microorganisms (CFU/ml) was determined. Results shown are the means and standard deviations from at least three to six independent experiments.
b
Values were significantly different (P < 0.05) from those obtained for A. baumannii exposed to control peptide or no peptide.