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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2016 Dec 12;16(2):323–333. doi: 10.1158/1535-7163.MCT-16-0501

Figure 4. Cytotoxic effect of c-CPE-NP encapsulating the p16 DT-A plasmid on ovarian tumor cells in vitro.

Figure 4

OSPC ARK-1 ovarian tumor cells were incubated with 500μg/ml of unconjugated NP, scr-NP or c-CPE-NP encapsulating the p16 DT-A plasmid for 72 hours in complete medium. After incubation cells were counted using the Trypan blue exclusion test as described in Methods. c-CPE-NP induced a significantly higher cytotoxic effect when compared with unconjugated NP and scr-NP. A similar toxic effect was observed following incubation of tumor cells with c-CPE-NP encapsulating the CMV DT-A plasmid (ie, positive control). In contrast, no significant differences in cytotoxicity were detected in HOSE cells (ie, claudin-3 and -4 negative).