FIG. 2.

Dose-dependent inhibition of HCV replicon by PI-1 or IFN-α alone. HCV replicon cells were treated with PI-1 (A) or IFN-α (B) for 48 h. At the end of the 48-h treatment, total cellular RNA was extracted with the RNeasy-96 kit. The levels of HCV replicon RNA remaining were then determined by quantitative RT-PCR, as described in Materials and Methods, and are shown as a percentage of the levels of replicon RNA in cells treated with 0.5% DMSO (control). Each bar represents the average of five cell culture replicates with the standard deviation.