FIG. 6.

Nine-day treatment of HCV replicon cells with PI-1, IFN-α, or ribavirin. The cells were treated with compound for 3, 6, or 9 days. At the end of each treatment period, cell numbers were determined by an MTS-based cell viability assay with an established standard curve, and the level of HCV RNA in the cells was determined by the quantitative RT-PCR assay. The copy number of HCV RNA per cell in each sample is plotted as the percentage (on a log scale) of the copy number in replicon cells incubated with 0.2% DMSO (control) for the same period of time. Each datum point represents the average of five cell culture replicates with the standard deviation.