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Effects of S-ISL on intracellular ROS levels: all groups were added with stimulant H2O2 (100 μM) except the vehicle group. The change of ROS levels in response to S-ISL (12.5~50 μg/mL) was examined using DCFH-DA analysis (a) and FCM analysis (b). S-ISL: synthetic isoliquiritigenin; ROS: reactive oxygen species; DCFH-DA: 2′, 7′- dichlorodihydrofluorescein diacetate; FCM: flow cytometry.
