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. Author manuscript; available in PMC: 2017 Feb 5.
Published in final edited form as: Chem Res Toxicol. 2016 Jul 5;29(12):1903–1911. doi: 10.1021/acs.chemrestox.6b00136

Figure 5.

Figure 5

Covalent binding profiles of liver microsomal protein fractions separated by two-dimensional electrophoresis. Loaded liver protein samples (100 μg) were subjected to isoelectric focusing (pI 3–10) and were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10–225 kDa). In vivo liver protein bindings with metabolically activated 14C-substrates in humanized liver mice were analyzed by accelerator mass spectrometry. The results for 5-n-butyl-pyrazolo[1,5-a]pyrimidine, a new drug candidate OT-7100 metabolite, are taken from Yamazaki et al.51