Fig. 3.
Curcumin decreases proliferation, sphere forming ability and colony forming potential in GSC cell lines. a Glio3 and Glio9 GSCs were plated at 1x105 cells initially and treated with 2.5 μM curcumin on day 0. Cells were counted using Orflo Technologies Cell Counter Moxi z on days 4, 7 and 10. b Glio3 GSCs were seeded at 50–100 cells per well in a 96-well plate and treated with 2.5 μM curcumin on day 0. Spheres were counted on day 14. c Glio9 GSCs were plated at 200 cells and treated with 2.5 μM curcumin at day 0. Colonies were stained with crystal violet and counted on day 14. *p < 0.05, non-treated controls (NT) vs. curcumin treated