. Author manuscript; available in PMC: 2017 Feb 5.

Published in final edited form as: J Appl Microbiol. 2014 Dec 2;118(1):92–98. doi: 10.1111/jam.12684

Table 1.

Comparison of flask fermentation with LB medium and three fed-batch fermentation experiments with different induction time points and feeding rates

	FB A	FB B	FB C	LB
OD₆₀₀ at induction time	7·5	9·5	3·5	0·6
Cell density at induction (g_cdw l⁻¹)	2·6	3·5	0·8
Feeding start time (h)	6	10	4
Fermentation time (h)	26	26	24
Maximum cell density (g_cdw l⁻¹)	7·6	10·5	5·7
Purified 6-OST-3 concentration (mg l⁻¹)	61·5	52·9	120·7	6·1
Yield 6-OST-3/biomass (mg g_cdw⁻¹)	8·1	5·0	21·2
Productivity 6-OST-3 (mg l⁻¹ h⁻¹)	2·4	2·0	5·0	0·29
Final specific activity (nmol min⁻¹ mg⁻¹)	1·36 ± 0·185	1·55 ± 0·144	1·67 ± 0·172	2·55
Productivity 6-OST-3 (U l⁻¹ h⁻¹)	3·26	3·10	8·35	0·74
NS6S content (% ± SD)	79·93 ± 2·45	82·94 ± 0·58	83·18 ± 3·07	93·68