FIG. 4.

Synergy at the 95% confidence level for the HCV-371 and PEGASYS combination study. Clone A cells were seeded in 96-well cell culture plates at subconfluent density in medium containing 2% FBS. Combinations of HCV-371 (0.3 to 30 μM) and PEGASYS (0.1 to 30 pg/ml) were added to the plates, forming a matrix. Dose-response curves for the individual drugs were run in duplicate. Plates were incubated for 72 h at 37°C and 5% CO₂. Total RNA was extracted from replicon-containing cells and analyzed for HCV RNA, 18S rRNA, and GAPDH RNA with a TaqMan RT-PCR. The resulting data were calculated at the 95% confidence level by MacSynergy II and displayed with DeltaGraph software.