Table 1.

Targeted genes and their primer sequences for SYBR Green and Methylation-Sensitive Real-Time Polymerase Chain Reaction.

Gene	Primer sequences
Mouse Foxp-3	Forward: 5′-CAGCTGCCTACAGTGCCCCTAG-3 Reverse: 5′-CATTTGCCAGCAGTGGGTAG-3′
Mouse Helios	Forward: 5′-TAAGCTCAGCTTATTCTCAGGTCTATCA-3′  Reverse: 5′-ATGTTGTTTTCGTGACTATCAGATGTT-3′
Mouse CTLA-4	Forward: 5′-CGCAGATTTATGTCATTGATCC-3′  Reverse: 5′-TTTTCACATAGACCCCTGTTGT-3′
Mouse TGF-β	Forward: 5′-AACAATTCCTGGCGTTACCTT-3′  Reverse: 5′-GAATCGAAAGCCCTGTATTCC-3′
Mouse IL-10	Forward: 5′-ACAGCCGGGAAGACAATAAC-3′  Reverse: 5′-CAGCTGGTCCTTTGTTTGAAAG-3′
Mouse Foxp3-TSDR	Forward: 5′-CTTGCTTCCTGGCACGAGATTTGAATTGGATATGGTTTGT-3′  Reverse: 5′-CAGGAAACAGCTATGACAACCTTAAACCCCTCTAACATC-3