TABLE 1.
C. coli strains tested in this study, PCR-RFLP results, and ciprofloxacin MICs for the corresponding strains
| Isolate no.a | Source/country of origin | gyrA mutation at codon 86b | Ciprofloxacin MIC (μg/ml) |
|---|---|---|---|
| CNET 051 | Human/France | Yes | 32 |
| CH 008 | Human/Spain | Yes | 32 |
| CH 024 | Human/Spain | No | 0.5 |
| CH 051 | Human/Spain | Yes | 32 |
| CH 056 | Human/Spain | No | 0.125 |
| CG001 | Human/Spain | Yes | 64 |
| CG002 | Human/Spain | Yes | 64 |
| CG003 | Human/Spain | No | 0.125 |
| CG004 | Human/Spain | Yes | 64 |
| CG005 | Human/Spain | Yes | 64 |
| CNET 019 | Flock outbreak/Netherlands | Yes | 16 |
| CNET 021 | Flock outbreak/Netherlands | Yes | 16 |
| CNET 066 | Pig/Netherlands | No | 0.25 |
| CNET 068 | Pig/North Ireland | No | 0.25 |
| CNET 069 | Pig/North Ireland | No | 0.25 |
| CNET 072 | Pig/Denmark | No | 0.25 |
| CNET 064 | Poultry/Denmark | No | 0.125 |
| CNET 082 | Poultry/North Ireland | No | 0.125 |
| CPM 011 | Poultry/Spain | Yes | 32 |
| CPM 021 | Poultry/Spain | Yes | 32 |
| CPM 023-1 | Poultry/Spain | Yes | 16 |
| CPM 042-7 | Poultry/Spain | Yes | 32 |
| CPM 043-3 | Poultry/Spain | Yes | 32 |
| CPM 043-4 | Poultry/Spain | Yes | 32 |
| CPM 045 | Poultry/Spain | Yes | 32 |
| CPM 053-3 | Poultry/Spain | Yes | 32 |
| CPM 057-1 | Poultry/Spain | Yes | 64 |
| CPM 058-2 | Poultry/Spain | Yes | 64 |
| CPM 063-1 | Poultry/Spain | Yes | 32 |
| CPM 063-4 | Poultry/Spain | Yes | 64 |
| CPM 064-2 | Poultry/Spain | No | 0.5 |
| CPM 069-2 | Poultry/Spain | Yes | 64 |
| CPM 072-1 | Poultry/Spain | Yes | 32 |
a
CNET, isolates from the CAMPYNET collection; CH and CG, isolates from humans with gastroenteritis; CPM, isolates from poultry samples at the retail level.
b
Mutants had a Thr-86-to-Ile (ACT-to-ATT) substitution.