Table 2.

Effects of DAT knock-down on striatal DAT and TH-immunoreactivity

	striatal DAT-immunoreactivity
	rostral		medial		caudal
Age	WT	DAT-KD	WT	DAT-KD	WT	DAT-KD
Pups (3–4 days)	1.00 ± 0.10	0.54 ± 0.05**	1.00 ± 0.10	0.57 ± 0.05**	1.00 ± 0.09	0.66 ± 0.07*
aged (18 months)	1.00 ± 0.09	0.42 ± 0.02**	1.00 ± 0.06	0.42 ± 0.04**	1.00 ± 0.11	0.41 ± 0.04**
	striatal TH-immunoreactivity
	rostral		medial		caudal
Age	WT	DAT-KD	WT	DAT-KD	WT	DAT-KD
Pups (3–4 days)	1.00 ± 0.05	1.03 ± 0.05	1.00 ± 0.05	1.10 ± 0.07	1.00 ± 0.04	1.16 ± 0.07
aged (18 months)	1.00 ± 0.11	0.94 ± 0.09	1.00 ± 0.11	0.98 ± 0.13	1.00 ± 0.13	0.88 ± 0.12

Striatal DAT and TH immunoreactivity. A coronal section of the rostral, medial and caudal striatum was used for each mouse (pups, WT n=7, DAT-KD =6; 18 months, WT=7, DAT-KD n=7). Data are represented as ratios to respective mean of WT (mean ± SEM) for comparison purposes only; all statistical analyses were performed on raw numbers (2×3 genotype x striatal subregion ANOVA for each age group, Holm-Sidak *p<0.05, **p<0.01 compared to respective WT). Tissue processing and immunohistochemistry for pups and aged mice were performed separately; therefore fluorescence intensities were not compared between ages. Intensity values were not saturated providing similar signal to noise ratio for all age groups.