Table 3.

Effects of DAT-KD and paraquat/maneb (PQ/MB) exposure on number of SNc neurons in 5 weeks old mice

region	group	TH+ neurons (n, Means ± SEM)	% loss (to saline)	neuron loss (compared to saline)	Total neurons (n, Means ± SEM)	% loss (to saline)	neuron loss (compared to saline)
SNc complete	WT_SAL	6212 ± 709			11291 ± 849
	WT_PQ/MB	3908 ± 309**	37	2310	7863 ± 808**	30	3428
	DAT_SAL	4483 ± 310^			8526 ± 528^^
	DAT_PQ/MB	3261 ± 508*	27	1222	7335 ± 333	14	1191
SNc medial	WT_SAL	1652 ± 152			2567 ± 255
	WT_PQ/MB	1073 ± 49**	35	579	1955 ± 141*	24	612
	DAT_SAL	1162 ± 97^^			1837 ± 147^^
	DAT_PQ/MB	592 ± 76**^^	49	570	1311 ± 149*^	29	526
SNc lateral	WT_SAL	1007 ± 181			2133 ± 273
	WT_PQ/MB	737 ± 100	27	270	1494 ± 281*	30	639
	DAT_SAL	658 ± 47^			1455 ± 101^^
	DAT_PQ/MB	531 ± 78	19	127	1420 ± 95	2	35
SNc ventral	WT_SAL	1303 ± 177			2383 ± 404
	WT_PQ/MB	718 ± 105**	45	585	1889 ± 182	21	494
	DAT_SAL	948 ± 83^			2113 ± 153
	DAT_PQ/MB	592 ± 110*	38	356	1821 ± 167	14	292
SNc dorsal	WT_SAL	2249 ± 356			4208 ± 466
	WT_PQ/MB	1381 ± 356	39	868	2524 ± 466*	40	1684
	DAT_SAL	1715 ± 251			3121 ± 330
	DAT_PQ/MB	1714 ± 301	0	1	3230 ± 394	0	0

Stereological estimates (means ± SEM) of TH-positive (TH+) neurons and TH-positive and –negative neurons (total neurons) in the SNc of 5 weeks old WT and DAT-KD mice injected with saline (WT_SAL n=5, DAT_SAL n=10) or PQ/MB (WT_PQ/MB n=5, DAT_PQ/MB n=7) as well as the percentage and the number of neurons lost compared to respective saline injected group (2×2 genotype x toxin between-subject ANOVA, Holm-Sidak *p<0.05, **p<0.01 compared to respective saline group; ^{^}p<0.05, ^{^^}p<0.01 compared to WT; significant interaction (p=0.04) of genotype x toxin in dorsal subregion for total neurons).