Table 4.
Effects of DAT-KD and paraquat/maneb (PQ/MB) exposure on number of SNc neurons in 11 months old mice
| region | group | TH+ neurons (n, Means ± SEM) | % loss (to saline) | neuron loss (compared to saline) | Total neurons (n, Means ± SEM) | % loss (to saline) | neuron loss (compared to saline) |
|---|---|---|---|---|---|---|---|
| SNc complete | WT_SAL | 3663 ± 393 | 10127 ± 755 | ||||
| WT_PQ/MB | 3632 ± 338 | 1 | 31 | 9260 ± 524 | 9 | 867 | |
| DAT_SAL | 3261 ± 243 | 8625 ± 586 | |||||
| DAT_PQ/MB | 3149 ± 370 | 3 | 112 | 8304 ± 1108 | 4 | 321 | |
| SNc medial | WT_SAL | 856 ± 77 | 2238 ± 109 | ||||
| WT_PQ/MB | 801 ± 51 | 6 | 55 | 2085 ± 182 | 7 | 153 | |
| DAT_SAL | 691 ± 79 | 2031 ± 185 | |||||
| DAT_PQ/MB | 552 ± 92 ^ | 20 | 139 | 1770 ± 182 | 13 | 261 | |
| SNc lateral | WT_SAL | 461 ± 82 | 1007 ± 131 | ||||
| WT_PQ/MB | 351 ± 88 | 24 | 110 | 944 ± 163 | 6 | 63 | |
| DAT_SAL | 428 ± 84 | 964 ± 146 | |||||
| DAT_PQ/MB | 523 ± 56 | 0 | 0 | 1258 ± 97 | 0 | 0 | |
| SNc ventral | WT_SAL | 560 ± 73 | 1738 ± 166 | ||||
| WT_PQ/MB | 554 ± 67 | 1 | 6 | 1772 ± 72 | 0 | 0 | |
| DAT_SAL | 545 ± 68 | 1580 ± 116 | |||||
| DAT_PQ/MB | 475 ± 89 | 13 | 70 | 1657 ± 131 | 0 | 0 | |
| SNc dorsal | WT_SAL | 1787 ± 283 | 5144 ± 498 | ||||
| WT_PQ/MB | 1925 ± 217 | 0 | 0 | 4460 ± 266 | 13 | 684 | |
| DAT_SAL | 1596 ± 156 | 4050 ± 377 | |||||
| DAT_PQ/MB | 1599 ± 170 | 0 | 0 | 3620 ± 908 | 11 | 430 |
Stereological estimates (means ± SEM) of TH-positive (TH+) neurons and TH-positive and –negative neurons (total neurons) in the SNc of 11 months old WT and DAT-KD mice injected with saline (WT_SAL n=5, DAT_SAL n=7) or PQ/MB (WT_PQ/MB n=6, DAT_PQ/MB n=9) as well as the percentage and the number of neurons lost compared to respective saline injected group (2×2 genotype x toxin between-subject ANOVA, Holm-Sidak, no effect of toxin; ^p<0.05 compared to respective WT).