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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Insect Biochem Mol Biol. 2016 Dec 7;81:19–31. doi: 10.1016/j.ibmb.2016.12.001

Figure 5. Developmental changes in fat body MsPLIN2 mRNA levels.

Figure 5

A) PLIN2 mRNA level was determined by qRT–PCR using mRNA RpS3 as reference. At least two independent sets of total RNA were analyzed in triplicate and relative values are plotted as the mean ± SD. D0, D1, D2, D3, D4 and D5 refers to days of 5th-instar larva, W3: wanderer day 3, P23: pupa day 23; M1 and F1: 1-day-old male and female adults, respectively. B) PLIN2 western blot: each gel lane was loaded with 10–20μg of LDs proteins. C) Ponceau S staining of membrane used in western blot shown in panel B.