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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Fertil Steril. 2016 Dec 8;107(2):439–447. doi: 10.1016/j.fertnstert.2016.11.008

Figure 3.

Figure 3

GPER mediates upregulation of HIF-1α and HIF-1α target gene expression induced by E2 and G1. ESCs were treated with E2 or G1, with or without pretreatment with G15 for 30min. (A) Evaluation of GPER protein expression by immunofluorescent microscopy in treated or untreated ESCs. (B) Immunoblots showing GPER and HIF-1α protein expression in ESCs under different treatment. (C, D) Quantitative comparison of the fold difference in GPER and HIF-1α protein expression. (E, F) Elisa assay showing VEGF and MMP9 secretion from ESCs of different conditions. (G, H) The transactivation of the VEGF and MMP9 promoters in ESCs by different treatment. (*P<0.05, **P<0.01, ***P<0.001 by ANOVA).