Figure 4. Protein expression profiles in response to TNFα in the absence or presence of TGFβ-induced EMT.
(A) Principle component analysis of the 4,398 proteins with abundance that are significantly different in at least one experiment group; from two biological replicates of hSAECs control cells (black open circle), hSAECs treated with TNFα (blue square), EMT-hSAECs control cells (red closed circle), and EMT-hSAECs treated with TNFa (purple diamond) are shown. (B) Heatmap of z score- and averaged log2-transformed ratios of the abundance of the 4,398 proteins with abundance that is significantly different in at least one experiment group (ANOVA, FDR 1%). Proteins were grouped using unsupervised hierarchical clustering. Examples of significantly enriched functional annotations for each cluster are shown (Fisher’s exact test, P<0.05, FDR <2%).