Fig. 6. Src homology 2 domain–containing adaptor protein B–knockdown (SHB^KD) dendritic cells (DCs) induce severe symptoms of atopic dermatitis in mice. Induction of atopic dermatitis was performed in BALB/c mice (n = 4) by applying dinitrocholorobenzene (Sigma) to the outer and inner surfaces of ears as described in the “Materials and Methods.” The mice were then subcutaneously injected twice on days 3 and 10 with 1×10⁶ OVA_323-339 peptide-pulsed SHB^KD or wild type (WT) mDCs. (A) Ears of mice with atopic dermatitis are shown after DC inoculation. (B) Ear thickness of mice with atopic dermatitis was monitored every 3 days and represented as the mean±standard deviation of 4 mice from each experiment. Student's t test. ^*p < 0.05, ^**p < 0.005. (C) Histological analysis of atopic dermatitis after inoculation with WT or SHB^KD DCs. The ears with dermatitis were obtained on day 33, fixed, dehydrated, and embedded in paraffin blocks. The histopathology of each paraffin section was examined after (× 400). Infiltrating immune cells are indicated by arrows.