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Poly(ADP-ribosyl)ation is enhanced by UV radiation and in parp triple knockout plants. PARylation of nuclear proteins was analyzed in 32-day-old leaf material using a poly(ADP-ribose) monoclonal antibody. The insert shows Ponceau staining of protein loaded onto the nitrocellulose membrane (1: Col-0 control, 2: parp1-1 parp2-1 parp3-1 control, 3: Col-0 UV-treated, 4: parp1-1 parp2-1 parp3-1 UV-treated). Error bars represent standard error (N = 3).
