FIGURE 2.

T-DNA insertion lines were identified for Arabidopsis thaliana PARP1 and PARP2. (A,C) Model of the genomic regions and the T-DNA insertions in PARP1 (A) and PARP2 (C). Coding regions are presented by white boxes; introns are shown by a line. Triangles indicate the sites of T-DNA insertions. The numbers indicate the last nucleotide before and the first nucleotide after the insertion, counting from the start condon. LB and RB indicate the left and right border of the T-DNA, as determined by sequencing. (B,D) RT-PCR analysis of leaf RNA showing the absence of full-length transcript of the respective PARP gene in the mutant lines. Actin2 served as a control.