FIGURE 1.

Effects of OSM in IL-2IC–treated CIA mice. DBA1/J mice were immunized intradermally with CII on day 0 and boosted by intradermal injection with CII on day 14. PBS (n = 10) or IL-2IC (n = 10) was injected i.p. into mice on day 0 (first injection), day 2 (second injection), and day 4 (third injection). (A) Arthritis severity was determined based on the mean arthritis index score and incidence score. (B) Confocal images of spleen tissues stained for CD4 (red), CD25 (blue), IL-17 (green), and Foxp3 (green). Positive cells are indicated in the graphs (right). (C) mRNA levels of OSM in spleen cells analyzed by real-time PCR. (D) Expression levels of STAT3, STAT5a, STAT5b, and SOCS3 in spleen cells were measured by real-time PCR. Data are presented as mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.005).