Fig. 5.

Overexpression of ephrinA3 downregulates serotonergic innervation in the amygdala and piriform cortex. A–B″, Electroporated neurons were revealed by TdTomato with the ephrinA3 plasmid in the targets of Am (A) and Pir (B). Corresponding 5-HT immunocytochemistry from the electroporated (A′, B′) and nonelectroporated (A″, B″) sides. Note the decrease of 5-HT fibers in the outlined area compared with control side. Scale bar = 100 μm. C, Target of electroporation (TdTomato) on a coronal section of P5 mouse brain at the level of amygdala. Arrows show electroporated Am and Pir. Scale bar = 1000 μm. D, E, Scattergram shows serotonergic fiber density in the electroporated (red) versus nonelectroporated (blue) amygdala (D) and piriform cortex (E). Overexpression of ephrinA3 resulted in a significant decrease of 5-HT fiber density compared with control groups (n = 5). Data are presented as mean ± SEM. Paired t test was used for ipsilateral vs. contralateral, ***p < 0.005.