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. 2017 Feb 6;8:85. doi: 10.3389/fimmu.2017.00085

Figure 3.

Figure 3

NF-κB inhibition attenuates toll-like receptor 2 and protease activated receptor 1 stimulated mediator release. Serotonin, sCD62P, RANTES, and sCD40L secretion levels from platelets stimulated by Pam3CSK4 (100 µg/ml) (A,C,E,G) or TRAP (50 µg/ml) (B,D,F,H) with (w) or without (w/o) the inhibitor of NF-κB (BAY 11-7082). Platelets were pre-treated (20 min, room temperature) with anti-human FcγRII monoclonal antibody IV.3 and incubated with inhibitor and stimulators as described in Section “Materials and Methods.” Levels of sCD62P (A,B), RANTES (C,D), sCD40L (E,F), and serotonin (G,H) were quantified by enzyme-linked immunosorbent assays (measured in triplicate). Background levels have been subtracted and the data (mean ± SD; n = 10 experiments, measured in triplicate) are expressed in picograms per milliliter. *P < 0.05; Mann–Whitney U test, unstimulated without inhibitor (BAY 11-7082) versus stimulated without inhibitor (BAY 11-7082) or unstimulated in the presence of inhibitor (BAY 11-7082, 2 μM) versus stimulated in the presence of inhibitor (BAY 11-7082, 2 μM). #P < 0.05 (Mann–Whitney U test); stimulated without inhibitor (BAY 11-7082) versus stimulated in the presence of inhibitor (BAY 11-7082, 2 μM).