Figure 2.

Time‐dependent transport of MPP⁺ through mOct2 (A), mOct3 (B), and mPmat (C). CHO‐K1 cells stably overexpressing mOct2, mOct3, or mPmat time‐dependently transported MPP⁺. Stable transformants and CHO‐K1 cells were incubated in the presence of 100 nm MPP⁺ for 2–60 min at 37 °C. Specific uptake in stable transformants is presented relative to that in mock‐transfected CHO‐K1 cells; Individual transport assays were performed in triplicate (three wells were analyzed for each group per trial). The results were confirmed at least two times in separate experiments using different cryopreserved cell vials. The results shown are representative of experiments performed.