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. 2017 Jan 9;7(2):237–248. doi: 10.1002/2211-5463.12183

Figure 3.

Figure 3

mOct2, mOct3, and mPmat activity dependence on extracellular Na+, Cl−, and pH. CHO‐K1 cells overexpressing mOct2 (A), mOct3 (B), or mPmat (C) were incubated in normal buffer, Na+‐free buffer, or Cl−‐free buffer containing 100 nm MPP+ for 5 min at 37 °C. MPP+ transport activities are expressed relative to those in normal buffer. CHO‐K1 cells overexpressing mOct2 (D), mOct3 (E), or mPmat (F) were incubated in pH‐modified buffers (pH 6.6, 7.4 or 8.2) with 100 nm MPP+ for 5 min at 37 °C. MPP+ transport activities are expressed relative to those in KRPH buffer at pH 7.4. Differences were identified using Student's t tests; *P < 0.05. Individual transport assays were performed in triplicate (three wells were analyzed for each group per trial). The results were confirmed at least two times in separate experiments using different cryopreserved cell vials. The results shown are representative of experiments performed.