Figure 5.

Effects of extracellular pH on neurotransmitters uptake activity of mOct2, mOct3, and mPmat. CHO‐K1 cells overexpressing mOct2 (A), mOct3 (B), and mPmat (C) were incubated in pH‐modified KRPH buffer (pH 6.6, 7.4 or 8.2) with 10 μm noradrenaline (A‐1, B‐1, C‐1), dopamine (B‐2, C‐2), serotonin (B‐3, C‐3), and histamine (A‐2, B‐4, C‐4) for 5 min at 37 °C. Transport activities are expressed relative to those in KRPH buffer at pH 7.4. Differences were identified using Student's t tests; *P < 0.05, **P < 0.01 and ***P < 0.001, respectively, Individual transport assays were performed in triplicate (three wells were analyzed for each group per trial). The results were confirmed at least two times in separate experiments using different cryopreserved cell vials. The results shown are representative of experiments performed.