Figure 8.

The effect of kinase inhibitors on the decynium‐22‐mediated OCT3 activation. We examined the effect of decynium‐22 on OCT3 transport activity in the presence of 10 μm protein kinase inhibitors: protein kinase A inhibitor H‐89 (A), protein kinase C inhibitor (RO‐32‐0432) (B), and Ca²⁺/Calmodulin‐dependent kinase II inhibitor KN‐93 (C). [³H] MPP⁺ was used as a substrate and the incubation time was 5 min. The amount of MPP⁺ transported in to CHO‐K1 cells without decynium‐22 was set to 1. Individual transport assays were performed in triplicate (three wells were analyzed for each group per trial). The results were confirmed at least two times in separate experiments using different cryopreserved cell vials. The results shown are representative of experiments performed.