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. 2017 Jan 18;7(2):174–186. doi: 10.1002/2211-5463.12172

Figure 3.

Figure 3

Sucralose‐induced signaling promotes S6 Ser 235/236 phosphorylation in an ERK1/2‐depenedent manner. (A–C) Sucralose activated ERK1/2 at 2–5 min and S6 pSer 235/236 at 30 min. Both events were abolished by pretreatment with the MEK1/2 inhibitor PD0325901 (A). Quantitation of immunoblots for pERK1/2/ERK1/2 (B) and S6 pSer 235/236 (C). Bar graph data are the mean ± SE. *P < 0.05 vs 0 min. **P < 0.05 vs DMSO control. (D–F) Pretreatment of MIN6 cells with RSK inhibitors FMK (2 μm) or BI‐D1870 (100 nm) (BI‐D) does not prevent sucralose‐induced S6 and ERK1/2 phosphorylation (D). Quantitation of immunoblots for pERK1/2/ERK1/2 (E) and S6 pSer 235/236 (F). Bar graph data are the mean ± SE. *P < 0.05 vs Basal. **P < 0.05 vs DMSO control.