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. 2017 Jan 18;7(2):174–186. doi: 10.1002/2211-5463.12172

Figure 4.

Figure 4

Sucralose activation of S6 is mTOR pathway‐independent. (A) MIN6 cells stimulated with sucralose, amino acids, or GLP‐1 in the absence or presence of rapamycin. (B–D) Relative quantitation of immunoblots from (A) for activation of p70 (B), S6 Ser 235/236 (C), and S6 Ser 240/244 (D). *P < 0.05 vs Basal. (E) MIN6 cells were treated with sucralose, amino acids, or GLP‐1, and p70 was immunoprecipitated. Kinase assay using GST‐S6 shows amino acids robustly activate p70, while sucralose does not. Relative quantitation of p70 activation by immunoblotting (F) and normalized radiolabel incorporation into GST‐S6 (G) is displayed as bar graphs of the mean ± SE. *P < 0.05 vs Basal.