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. 2017 Jan 18;7(2):174–186. doi: 10.1002/2211-5463.12172

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© 2016 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Sucralose, GLP‐1, and cAMP analogs each induce S6 phosphorylation at Ser235/236. (A) MIN6 cells were stimulated with either 6‐BNZ or ESCA to activate PKA or EPAC, respectively for the indicated times. (B) MIN6 cells stimulated with sucralose, GLP‐1, or combinations of PKA and EPAC activators. S6 Ser 235/236 and Ser 240/244 activation was monitored by immunoblotting. Relative quantitation of the phospho‐S6 immunoblots are shown in (C) and (D). Bar graphs are the mean ± SE. *P < 0.05 vs Basal.