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. 2017 Jan 17;7(2):160–173. doi: 10.1002/2211-5463.12173

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© 2016 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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pSer25AnxA2 and pTyr23AnxA2 display distinct subcellular distributions in PC12 cells. Immunofluorescence double‐staining was carried out using rabbit polyclonal antibodies against pSer25AnxA2 (red) and mouse monoclonal antibodies against pTyr23AnxA2 (green), followed by secondary anti‐rabbit and anti‐mouse antibodies coupled to Alexa 594 (red fluorescence) and fluorescein isothiocyanate (FITC) (green fluorescence) respectively. DNA staining with 4′,6‐diamidino‐2‐phenylindole (blue fluorescence) was used to visualise the nucleus. Note that pTyr23AnxA2 (green) is present as a punctate pattern in both the nucleus and the cytoplasm, while pSer25AnxA2 (red) is predominantly found in large punctate structures in the cytoplasm and next to the NE (arrows). Scale bar is 10 μm.