Figure 1.

HSV‐1 gD‐induced signalling in human primary macrophages. Monocyte‐derived macrophages were stimulated with HSV‐1 gD (1 μg·mL⁻¹) for the indicated times. Following cell lysis, proteins were separated by SDS/PAGE and subjected to western blotting with antibodies to I‐κB, phospho‐NF‐κB p65 (P‐p65), phospho‐ERK1/2 (P‐ERK1/2) and phospho‐Akt (P‐Akt). Parallel immunoblotting with antibodies to NF‐κB p65 subunit, GAPDH, ERK1/2 and Akt confirmed equal loading of samples. Data are representative of three separate experiments conducted with cells from distinct donors.