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. 2017 Feb 6;7:42059. doi: 10.1038/srep42059

Figure 4. The −1243 to −1212 fragment of the −1323 to −1212 region is the core R. solani-responsive region.

Figure 4

(a) Schematic diagram of the −1323 to −1212 region (pCXGFP delA) and the four deleted derivatives (pCXGFP delB to pCXGFP delE) used to express GFP in tobacco leaves. The white boxes represent the 35S minimum promoter. (b) GFP fluorescence assay of young and expanded symmetrical tobacco leaves infiltrated with pCXGFP delA or its derivatives after R. solani strain YWK196 infection for 24 h. GFP fluorescence was observed under a Leica M205 C stereo microscope. Bars = 5 mm. (c) Quantitative fluorometric assay of tobacco leaves prepared in (b). The fluorescence value was calculated relative to CK of pCXGFP delA. The 35S minimum promoter was used as the negative control. Error bars indicate the SD (n = 3). Asterisks indicate P < 0.05 (*) in Student’s t test analysis.