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. 2017 Feb 6;7:42059. doi: 10.1038/srep42059

Figure 6. The GTTGA (−1243 to −1239) and TATTT (−1232 to −1228) sequences are two novel cis-elements confer induction by R. solani.

Figure 6

(a) Schematic diagram of the 16 bp sequence (I) and the two deleted derivatives (II and III) used to express GFP in tobacco leaves. The white boxes represent the 35S minimum promoter. (b) GFP fluorescence assay of young and expanded symmetrical tobacco leaves infiltrated with the three constructs prepared in (a) after R. solani strain YWK196 infection for 24 h. GFP fluorescence was observed under a Leica M205 C stereo microscope. Bars = 5 mm. (c) Quantitative fluorometric assay of tobacco leaves prepared in (b). Fluorescence value was calculated relative to CK of I. The 35S minimum promoter was used as the negative control. Error bars indicate the SD (n = 3). Asterisks indicate P < 0.05 (*) in Student’s t test analysis.