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. 2017 Feb 6;7:41633. doi: 10.1038/srep41633

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Volumetric bead distribution in the green channel, (b) Beads bound on the surface in red channel, (c) HuO9 labeled with the lipophilic dye DiD and (d) Image composite of the two bead layers in orthogonal view (x-z axis). (e–h) Absolute displacements in μm, analyzed using PIV (e,g) and KLT (f,h) for the volume beads (e,f) and surface beads (g,h). (i–l) Absolute Traction fields in Pa as obtained using PIV displacements (i,k) and KLT (j,l) for volume beads (i,j) and surface beads (k,l). Scale bar represents 30 μm.