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. 2017 Feb 6;7:41633. doi: 10.1038/srep41633

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Up to a bead density of 5 beads/μm², the error decreases significantly. A bead density higher than 5 beads/μm² only marginally decreases DTM, but also the SNR. All displacements were calculated using KLT with a window size of 8 × 8 pixels.