Table 1. Overview of the simulation parameters used in this work.
Parameter | Value range |
---|---|
Substrate Thickness | 100 μm |
Substrate mechanical properties | E = 20 kPa, v = 0.49 |
Simulated Traction | 5–50% E |
FA diameter size | [0.5, 0.75, 1, 2, 3, 4, 5] μm |
Bead density | [0.33, 1, 5, 10] Beads/(μm3) |
Bead type | 0.2 μm red-fluorescent beads (emission: 605 nm) |
Bead location | 3D (volumetric), 2D (surface) |
Microscope simulation settings | Spinning-Disk Confocal with 60 × 1.4 Oil immersion lens. Pixel size (x, y, z) = [0.06 0.06 0.25] μm, ni = 1.51, ns = 1.4, NA = 1.4 |
Sample size | N = 20 |