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. 2017 Feb 6;7:42195. doi: 10.1038/srep42195

Figure 3. HC-HA/PTX3 inhibits interstitial fibrosis in lacrimal gland of cGVHD mice.

Figure 3

Lacrimal glands harvested at Day 32 after BMT, i.e., 4 days after the last injection of PBS or HC-HA/PTX3, were sectioned for H & E (a,b), Mallory (c,d) staining (Scale bar = 100 μm), immunofluorescence staining of HSP47 (green) (e,i and f), Scale bar = 50 μm, G, H, and J, Scale bar = 20 μm), quantitative comparison of the number of HSP47+ fibroblasts per field (k), **p = 0.006 for HSP47, N = 8 for PBS and N = 12 for HC-HA/PTX3). Transcript levels of each molecules, p = 0.006 for HSP47(L), p = 0.004 for collagen type Iα1 (m), *p = 0.033 for collagen type IIIα1 (n), not statistically significant for TGF-β1 (o) *p = 0.038 for NF-kB (p) by quantitative real-time PCR analyses using the expression of GAPDH as the internal control (*p < 0.05, **p < 0.01, N = 5 for PBS and N = 12 for HC-HA/PTX3). The HSP47+ cells per field (at x 400 magnification) were counted in at least 5 different fields on 3–5 sections.