Figure 5. HC-HA/PTX3 suppresses inflammation and fibrosis in conjunctiva of cGVHD mice.

Conjunctiva harvested at Day 32 after BMT, i.e., 4 days after the last injection of PBS or HC-HA/PTX3, were sectioned for H & E (a and b), Scale bar = 100 μm) and Mallory staining (c and d), Scale bar = 100 μm) and immunostaining for CD45⁺ cells (red) (e and f), MHC class II⁺ cells (red) (g and h) and HSP47⁺ activated fibroblasts (green) (i and j) for both groups (Scale bar = 50 μm for (e–h). The number of HSP47⁺ cells/field was compared between the two groups (**p = 0.005, N = 4 for PBS and N = 4 for HC-HA/PTX3).