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. 2017 Feb 6;7:42138. doi: 10.1038/srep42138

Figure 1. Assessment of pluripotency phenotype by conventional markers following exposure to ROCK inhibitor.

Figure 1

(a) Experimental design. hPSCs were pre-treated with 10 μΜ Y-27236 ROCK inhibitor for 1–2 hours. Then, colonies were dissociated into single cells and exposed to 10 μΜ Y-27236 ROCK inhibitor. Samples were collected at five time-points: 0 h were untreated with cells remained in colonies whereas single cells were sampled at all other time-points (12 h, 24 h, 48 h and 96 h). (b) hPSC cell phenotype by flow cytometry and qRT-PCR was maintained for at least 48 hours following exposure to ROCK inhibitor, with reduction in stemness phenotype noted by 96 hours. *p < 0.05.