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. 2017 Feb 6;7:42097. doi: 10.1038/srep42097

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The ITS HRM assay is performed on DNA extracted from clinical samples such as positive blood culture samples. Our novel classification algorithm allows generated melt curves to identify the bacterial pathogens by matching against a reference database. Same-colored dots represent replicates from the same species classified within their boundaries and black dots outside all boundaries represent replicates from species that is not in our reference database, whereas the unknown species in the test sample (X) is identified by the classification algorithm.