Skip to main content
. 2017 Feb 6;7:38105. doi: 10.1038/srep38105

Figure 6. Antioxidants inhibit K562 cell erythroid differentiation enhanced by lactic acid.

Figure 6

(a–d) K562 cells were treated with combinations of 10-mM lactic acid, 50-μM β-ME, and 50 U SOD. (a) K562 cells were analysed via flow cytometry for ROS production 6 hours after lactic acid, SOD and β-ME treatments. The relative mean fluorescence intensity (MFI) of ROS was calculated relative to the values of the normal group. *P < 0.05. (b) Real time RT-PCR was used for haemoglobin and CD235a gene expression analyses at 72 hours. mRNA levels were normalized to the expression of β-actin mRNA. *P < 0.05. (c,d) Antioxidants (SOD and β-ME) blocked the K562 erythroid differentiation promoted by 10-mM lactic acid in vitro. (c) Haemoglobin levels in the K562 cells were detected via immunoblotting 72 hours after lactic acid and antioxidant treatment. The bands were quantified using Quantity One software, and the β-actin bands were used for normalization. (d) Flow cytometry was used to detect the CD235a expression levels in the K562 cells 72 hours after lactic acid and antioxidant treatment. The relative MFI of CD235a were calculated normalized to the values of non stained cells. The data represent the mean ± S.E.M. *P < 0.05.