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| Gene region | COI | Cytb | EF | |
|---|---|---|---|---|
| Primer pair | LCO1490puc-HCO2198puc | UEA3-HCO2198 | CB1-CB2 | F2-557F-F2-1118R |
| “COI-long” | “COI-short” | |||
| Primer position |  |
|||
| Amplicon size (nt) | 658 | 409 | 433 | 518 |
| MiSeq sequenced product (nt)* | 709 | 460 | 485 | 563 |
| Read overlap? | no | yes | yes | no |
(*Sequenced product = forward primer + amplicon + reverse primer).
