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. 2017 Feb 6;7:41948. doi: 10.1038/srep41948

Table 1. DNA regions targeted for amplification.

Gene region COI Cytb EF
Primer pair LCO1490puc-HCO2198puc UEA3-HCO2198 CB1-CB2 F2-557F-F2-1118R
  COI-long” COI-short”    
Primer position Inline graphic    
Amplicon size (nt) 658 409 433 518
MiSeq sequenced product (nt)* 709 460 485 563
Read overlap? no yes yes no

(*Sequenced product = forward primer + amplicon + reverse primer).